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LearnA700-085
antibody from Invitrogen Antibodies
Targeting: CDK1
CDC2, CDC28A
Western blot Immunocytochemistry
Immunoprecipitation Immunohistochemistry Flow cytometry Other assayAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
- Flow cytometry [1]
- Other assay [1]
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- Product number
- A700-085 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CDK1 Recombinant Rabbit Monoclonal Antibody (BLR085G)
- Antibody type
- Monoclonal
- Antigen
- Other
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Antibody clone number
- BLR085G
- Vial size
- 100 µL
- Concentration
- 100 µg/mL
- Storage
- 4° C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of mouse CDK1 by western blot. Samples: Whole cell lysate (50 µg) from BW5147.3 and F9 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-CDK1 recombinant monoclonal antibody [BLR085G] (Product # A700-085 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG (A120-101P). Chemiluminescence with an exposure time of 3 minutes. Lower Panel: Rabbit anti-Actin (Product # A300-491A).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human CDK1 by western blot. Samples: Whole cell lysate (50 µg) from HeLa, U2OS, K-562, HEK293T, A-549, Jurkat, MCF-7, and RKO cells prepared using NETN lysis buffer. Antibody: Rabbit anti-CDK1 recombinant monoclonal antibody [BLR085G] (Product # A700-085 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG (A120-101P). Chemiluminescence with an exposure time of 30 seconds. Lower Panel: Rabbit anti-Actin (Product # A300-491A).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human CDK1 in FFPE MOLT4 cells by ICC. Antibody: Rabbit anti-CDK1 recombinant monoclonal antibody [BLR085G] (Product # A700-085 lot 1). Secondary: HRP-conjugated goat anti-rabbit IgG (A120-501P). Substrate: DAB.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human CDK1 in FFPE breast carcinoma by IHC. Antibody: Rabbit anti-CDK1 recombinant monoclonal antibody (BLR085G) (A700-085 lot 1). Secondary: HRP-conjugated goat anti-rabbit IgG (A120-501P). Substrate: DAB.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human CDK1 in FFPE prostate carcinoma by IHC. Antibody: Rabbit anti-CDK1 recombinant monoclonal antibody [BLR085G] (Product # A700-085 lot 1). Secondary: HRP-conjugated goat anti-rabbit IgG (A120-501P). Substrate: DAB.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human CDK1 (shaded) in Jurkat cells by flow cytometry. Antibody: Rabbit anti-CDK1 recombinant monoclonal [BLR085G] (Product #A700-085; lot 1) or isotype control (unshaded). Secondary: DyLight® 488-conjugated goat anti-rabbit IgG (Product # A120-101D2).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human CDK1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Rabbit anti-CDK1 recombinant monoclonal antibody [BLR085G] (Product # A700-085 lot 1) used for IP at 20 µL/mg lysate. CDK1 was also immunoprecipitated by rabbit anti-CDK1 antibodies (Product # A303-633A and Product # A303-634A). For blotting immunoprecipitated CDK1 (Product # A700-085) was used at 1:1000. Chemiluminescence with an exposure time of 10 seconds.
