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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
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- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Flow cytometry [1]
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- Product number
- MA5-15062 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Phospho-CDK1 (Tyr15) Monoclonal Antibody (E.658.6)
- Antibody type
- Monoclonal
- Antigen
- Synthetic peptide
- Description
- It is not recommended to aliquot this antibody.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Antibody clone number
- E.658.6
- Vial size
- 100 µL
- Concentration
- 13 µg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot was performed using Phospho-CDK1 (Tyr15) Monoclonal Antibody (E.658.6) (Product # MA5-15062) and a 34 kDa band corresponding to CDK1 phosphorylated at Tyr15 was observed. Western blot analysis (Fig. a) was performed using whole cell extracts (30 µg lysate) of HeLa (Lane 1) and HeLa treated with Hydroxyurea (4 mM, 20 hours) (Lane 2) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0322BOX), 12 well. Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:20,000) using the iBright™ FL1500 Imaging System (Product # A44115). Chemiluminescent detection was performed using SuperSignal™ West Pico PLUS Chemiluminescent Substrate (Product # 34580). Densitometric analysis of this western blot is shown in histogram (Fig. b). Upregulation of CDK1 phosphorylation at Tyr15 is observed upon Hydroxyurea treatment.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of Phospho-Cdc2 pTyr15 in asynchronous HeLa cells using a Phospho-Cdc2 pTyr15 monoclonal antibody (Product # MA5-15062) (green) and a Phospho-Histone H3 monoclonal antibody (red).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometric analysis of Phospho-Cdc2 pTyr15 in Jurkat cells using a Phospho-Cdc2 pTyr15 monoclonal antibody (Product # MA5-15062) versus propidium iodide (DNA content).
