Antibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [5]
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Validation data
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- Product number
- NBP1-85729 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-85729, RRID:AB_11008973
- Product name
- Rabbit Polyclonal CDC2/CDK1 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Specificity of human, mouse, rat CDC2/CDK1 antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references An inverse switch in DNA base excision and strand break repair contributes to melphalan resistance in multiple myeloma cells.
Commercial Cdk1 antibodies recognize the centrosomal protein Cep152.
Clinically relevant progestins regulate neurogenic and neuroprotective responses in vitro and in vivo.
Sousa MM, Zub KA, Aas PA, Hanssen-Bauer A, Demirovic A, Sarno A, Tian E, Liabakk NB, Slupphaug G
PloS one 2013;8(2):e55493
PloS one 2013;8(2):e55493
Commercial Cdk1 antibodies recognize the centrosomal protein Cep152.
Lukinavičius G, Lavogina D, Gönczy P, Johnsson K
BioTechniques 2013 Sep;55(3):111-4
BioTechniques 2013 Sep;55(3):111-4
Clinically relevant progestins regulate neurogenic and neuroprotective responses in vitro and in vivo.
Liu L, Zhao L, She H, Chen S, Wang JM, Wong C, McClure K, Sitruk-Ware R, Brinton RD
Endocrinology 2010 Dec;151(12):5782-94
Endocrinology 2010 Dec;151(12):5782-94
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: CDC2/CDK1 Antibody [NBP1-85729] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: CDC2/CDK1 Antibody [NBP1-85729] - Analysis in human cell line A-431.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: CDC2/CDK1 Antibody [NBP1-85729] - Staining of human pancreas shows no cytoplasmic positivity in exocrine glandular cells as expected.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: CDC2/CDK1 Antibody [NBP1-85729] - Staining of human testis shows moderate nuclear positivity in cells in seminiferous ducts.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: CDC2/CDK1 Antibody [NBP1-85729] - Staining of human tonsil shows moderate cytoplasmic positivity in germinal center cells.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: CDC2/CDK1 Antibody [NBP1-85729] - Staining of human skin shows moderate cytoplasmic positivity in epidermal cells.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: CDC2/CDK1 Antibody [NBP1-85729] - Staining in human tonsil and pancreas tissues . Corresponding CDK1 RNA-seq data are presented for the same tissues.