MA5-15631

antibody from Invitrogen Antibodies

Targeting: CDK1 CDC2, CDC28A

Antibody data

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Validation data

Product number: MA5-15631 - Provider product page
Provider: Invitrogen Antibodies
Product name: CDK1 Monoclonal Antibody (8C5A7F10)
Antibody type: Monoclonal
Antigen: Purifed from natural sources
Description: MA5-15631 targets CDC2 in FACS, IF, and WB applications and shows reactivity with Human samples.
Antibody clone number: 8C5A7F10
Concentration: Conc. Not Determined

CDK1 protein structure - MA5-15631 shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of CDC2 using CDC2 monoclonal antibody (Product # MA5-15631) in Jurkat (1) cell lysate.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of CDC2 using CDC2 monoclonal antibody (Product # MA5-15631) in Jurkat (1) cell lysate.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Knockdown of CDK1 was achieved by transfecting HeLa with CDK1 specific siRNAs (Silencer® select Product # S465, S464). Western blot analysis (Fig. a) was performed using whole cell extracts from the CDK1 knockdown cells (lane 3), non-targeting scrambled siRNA transfected cells (lane 2) and untransfected cells (lane 1). The blot was probed with CDK1 Monoclonal Antibody (8C5A7F10) (Product # MA5-15631, 1:2000) and Goat anti-Mouse IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A28177, 1:20,000). Densitometric analysis of this western blot is shown in histogram (Fig. b). Decrease in signal upon siRNA mediated knock down confirms that antibody is specific to CDK1.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot was performed using CDK1 Monoclonal Antibody (8C5A7F10) (Product # MA5-15631) and a 34 kDa band corresponding to CDK1 was observed. Whole cell extracts (30 µg lysate) of HeLa (Lane 1), A549 (Lane 2), Hep G2 (Lane 3) and HEK-293 (Lane 4) were electrophoresed using NuPAGE™ 10% Bis-Tris Protein Gel (Product # NP0301BOX), 10 well. Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:2000) and detected by chemiluminescence with Goat anti-Mouse IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A28177, 1:20,000) using the iBright™ FL1500 Imaging System (Product # A44115). Chemiluminescent detection was performed using SuperSignal™ West Pico PLUS Chemiluminescent Substrate (Product # 34580).