Explore
Validate
Learn
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [2]
Submit
Validation data
Reference
Comment
Report error
- Product number
- PA1-4617 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Phospho-CDK1 (Tyr15) Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- This antibody is predicted to react with mouse and zebrafish based on 100% sequence homology.
Submitted references Iron-independent phosphorylation of iron regulatory protein 2 regulates ferritin during the cell cycle.
Wallander ML, Zumbrennen KB, Rodansky ES, Romney SJ, Leibold EA
The Journal of biological chemistry 2008 Aug 29;283(35):23589-98
The Journal of biological chemistry 2008 Aug 29;283(35):23589-98
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot of SK-N-MC total cell extracts, treated with hydroxyurea for 22 hours to induce Tyr15 cdc2 phosphorylation (Control). The immunolabeling is blocked by the phosphopeptide used as the antigen (Peptide Block) but not by the corresponding dephosphopeptide (not shown).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot of CDK1 in human T47D cells showing specific immunolabeling of a band at ~34 kDa corresponding to Phospho-CDK1 (Tyr15) polyclonal antibody (Product # PA1-4617) (Control, Lane 1). Treatment with EGF (30 ng per mL for 30 min) caused dephosphorylation of the Tyr15 on cdc2 (Lane 2). Phosphospecificity is shown in lanes 3 and 4, where the immunolabeling is completely eliminated by blot treatment with lambda phosphatase (1,200 units for 30 min).
