711228

antibody from Invitrogen Antibodies

Targeting: IGFBP2 IBP2

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

711228

Provider

Invitrogen Antibodies

Product name

IGFBP2 Recombinant Polyclonal Antibody (1HCLC)

Antibody type

Polyclonal

Antigen

Other

Description

This antibody is predicted to react with Monkey, Bovine, Pig, Sheep and Goat

Antibody clone number

1HCLC

Concentration

0.5 mg/mL

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis was performed on Whole cell extracts (30 µg lysate) of T-47D (Lane 1), T-47D (Condition Media) (Lane 2), PC-3 (Lane 3), PC-3 (Condition Media) (Lane 4), HeLa (Lane 5), HeLa (Condition Media) (Lane 6), SH-SY5Y (Lane 7), SH-SY5Y (Condition Media) (Lane 8). The blots were probed with Anti-IGFBP2 Recombinant Rabbit Polyclonal Antibody (Product # 711228, 1-2 µg/mL) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal Secondary Antibody, HRP conjugate (Product # A27036, 0.4 µg/mL, 1:2500 dilution). A 50 kDa Glycosylated and 35 kDa band for condition media corresponding to IGFBP2 was observed across the cell lines tested. Known quantity of protein samples were electrophoresed using Novex®NuPAGE®4-12% Bis-Tris gel (Product # NP0321BOX), XCell SureLock Electrophoresis System (Product # EI0002) and Novex® Sharp Pre-Stained Protein Standard (Product # LC5800). Resolved proteins were then transferred onto a nitrocellulose membrane with iBlot® Dry Blotting System (Product # IB21001). The membrane was probed with the relevant primary and secondary Antibody following blocking with 5% skimmed milk. Chemiluminescent detection was performed using Pierce™ ECL Western blotting Substrate (Product # 32106).