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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Western blot [2]
- Immunocytochemistry [2]
- Immunohistochemistry [1]
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- Product number
- PA5-85534 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- IGFBP2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Keep as concentrated solution. Predicted reactivity: Mouse (83%), Rat (82%), Pig (91%), Sheep (84%), Bovine (87%). Positive Control: SK-N-SH. Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of IGFBP2 in A) SK-N-AS whole cell lysate using IGFBP2 polyclonal antibody (Product # PA5-85534) using 30 µg of sample at a dilution of 1:1000. Prior to incubation with primary antibody, the sample was separated on 10% SDS-PAGE.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- IGFBP2 Polyclonal Antibody detects IGFBP2 protein by western blot analysis. A. 30 µg SK-N-AS whole cell lysate/extract.10 % SDS-PAGE. IGFBP2 Polyclonal Antibody (Product # PA5-85534) dilution: 1:1,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- IGFBP2 Polyclonal Antibody detects IGFBP2 protein at cytoplasm by immunofluorescent analysis. Sample: Huh-7 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: IGFBP2 protein stained by IGFBP2 Polyclonal Antibody (Product # PA5-85534) diluted at 1:500. Blue: Hoechst 33342 staining.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- IGFBP2 Polyclonal Antibody detects IGFBP2 protein at cytoplasm by immunofluorescent analysis. Sample: Huh-7 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: IGFBP2 protein stained by IGFBP2 Polyclonal Antibody (Product # PA5-85534) diluted at 1:500. Blue: Hoechst 33342 staining.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- IGFBP2 Polyclonal Antibody detects IGFBP2 protein at cytosol on human breast carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human breast carcinoma. IGFBP2 Polyclonal Antibody (Product # PA5-85534) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
