Antibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Western blot [2]
- Blocking/Neutralizing [1]
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Validation data
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- Product number
- AF674 - Provider product page
- Provider
- R&D Systems
- Product name
- Human IGFBP-2 Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects human IGFBP-2 in direct ELISAs and Western blots.
- Reactivity
- Human
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
CAA34373
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Insulin-like growth factor-binding protein 2 secreted by a tumorigenic cell line supports ex vivo expansion of mouse hematopoietic stem cells.
The role of matrix metalloproteinase-7 in redefining the gastric microenvironment in response to Helicobacter pylori.
Huynh H, Iizuka S, Kaba M, Kirak O, Zheng J, Lodish HF, Zhang CC
Stem cells (Dayton, Ohio) 2008 Jun;26(6):1628-35
Stem cells (Dayton, Ohio) 2008 Jun;26(6):1628-35
The role of matrix metalloproteinase-7 in redefining the gastric microenvironment in response to Helicobacter pylori.
McCaig C, Duval C, Hemers E, Steele I, Pritchard DM, Przemeck S, Dimaline R, Ahmed S, Bodger K, Kerrigan DD, Wang TC, Dockray GJ, Varro A
Gastroenterology 2006 May;130(6):1754-63
Gastroenterology 2006 May;130(6):1754-63
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human IGFBP-2 by Western Blot. Western blot shows lysates of T47D human breast cancer cell line. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human IGFBP-2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF674) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for IGFBP-2 at approximately 35 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human IGFBP-2 by Simple WesternTM. Simple Western lane view shows lysates of T47D human breast cancer cell line, loaded at 0.2 mg/mL. A specific band was detected for IGFBP-2 at approximately 42 kDa (as indicated) using 25 µg/mL of Goat Anti-Human IGFBP-2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF674) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- IGFBP-2 Inhibition of IGF-II-dependent Cell Proliferation and Neutralization by Human IGFBP-2 Antibody. Recombinant Human IGFBP-2 (Catalog # 674-B2) inhibits Recombinant Human IGF-II (Catalog # 292-G2) induced proliferation in the MCF-7 human breast cancer cell line in a dose-dependent manner (orange line). Inhibition of Recombinant Human IGF-II (14 ng/mL) activity elicited by Recombinant Human IGFBP-2 (0.2 µg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human IGFBP-2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF674). The ND50 is typically 2.5-7.5 µg/mL.