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Western blot
Blocking/NeutralizingAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [3]
- ELISA [1]
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- Product number
- H00008904-M01 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#H00008904-M01, RRID:AB_2084208
- Product name
- Mouse Monoclonal CPNE1 Antibody
- Antibody type
- Monoclonal
- Description
- IgG purified. CPNE1 - copine I
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: CPNE1 Antibody (8B8) [H00008904-M01] - Analysis of CPNE1 over-expressed 293 cell line, cotransfected with CPNE1 Validated Chimera RNAi ( Cat # H00008904-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with CPNE1 monoclonal antibody (M01), clone 8B8 (Cat # H00008904-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: CPNE1 Antibody (8B8) [H00008904-M01] - Analysis of CPNE1 expression in transfected 293T cell line by CPNE1 monoclonal antibody (M01), clone 8B8.Lane 1: CPNE1 transfected lysate(59.1 KDa).Lane 2: Non-transfected lysate.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: CPNE1 Antibody (8B8) [H00008904-M01] - CPNE1 monoclonal antibody (M01), clone 8B8 Analysis of CPNE1 expression in HeLa.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Sandwich ELISA: CPNE1 Antibody (8B8) [H00008904-M01] - Detection limit for recombinant GST tagged CPNE1 is approximately 1ng/ml as a capture antibody.
