PA5-117887

antibody from Invitrogen Antibodies

Targeting: CPNE1 CPN1

Western blot (Data presented on provider website)

ELISA (Recommended by provider)

Immunoprecipitation (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-117887

Provider

Invitrogen Antibodies

Product name

CPNE1 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

100 μL

Storage

-20°C, Avoid Freeze/Thaw Cycles

Immunoprecipitation

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

CPNE1 Immunoprecipitation using: Lane A: 0.5 mg Hela Whole Cell Lysate, Lane B: 0.5 mg HepG2 Whole Cell Lysate 2 µL with CPNE1 Polyclonal Antibody (Product # PA5-117887) and 60 μg of Immunomagnetic beads Protein G. Primary antibody: CPNE1 Polyclonal Antibody, at 1:100 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5,000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 59 kDa. Observed band size: 59 kDa.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunoprecipitation of CPNE1 in Lane A: 0.5 mg Hela Whole Cell lysate, Lane B: 0.5 mg HepG2 Whole Cell Lysate. Samples were treated with 60 μg of immunomagnetic Protein G beads, incubated with polyclonal antibody (Product # PA5-117887) with a dilution of 1:100 , followed by Dylight 800-labeled antibody to rabbit IgG (H+L) using a dilution of 1:5,000. Assay was performed under reducing conditions. Predicted band size: 59 kDa, Observed band size: 59 kDa .

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunoprecipitation of CPNE1 in Lane A: 0.5 mg Hela Whole Cell lysate, Lane B: 0.5 mg HepG2 Whole Cell Lysate. Samples were treated with 60 μg of immunomagnetic Protein G beads, incubated with polyclonal antibody (Product # PA5-117887) with a dilution of 1:100 , followed by Dylight 800-labeled antibody to rabbit IgG (H+L) using a dilution of 1:5,000. Assay was performed under reducing conditions. Predicted band size: 59 kDa, Observed band size: 59 kDa .

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

CPNE1 Immunoprecipitation using: Lane A: 0.5 mg Hela Whole Cell Lysate, Lane B: 0.5 mg HepG2 Whole Cell Lysate 2 µL with CPNE1 Polyclonal Antibody (Product # PA5-117887) and 60 μg of Immunomagnetic beads Protein G. Primary antibody: CPNE1 Polyclonal Antibody, at 1:100 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5,000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 59 kDa. Observed band size: 59 kDa.