F55012

antibody from NSJ Bioreagents

Targeting: CDC37 P50CDC37

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

F55012

Provider

NSJ Bioreagents

Product name

Cdc37 Antibody

Antibody type

Polyclonal

Description

This highly specific Cdc37 antibody is suitable for use in Flow cytometry/Immunohistochemistry/Western blot applications with human and rat samples.

Reactivity

Human, Rat

Host

Rabbit

Conjugate

Unconjugated

Vial size

0.08 ml, 0.4 ml

Concentration

In 1X PBS, pH 7.4, with 0.09% sodium azide

Storage

Aliquot the Cdc37 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Western blot testing of 1) human A431, 2) rat C6, 3) human Jurkat, 4) human K562 and 5) human LNCaP cell lysate with Cdc37 antibody. Expected molecular weight: 44-50 kDa.

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Western blot testing of human 1) MCF7 and 2) A2058 cell lysate with Cdc37 antibody. Expected molecular weight: 44-50 kDa.

Immunohistochemistry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC testing of FFPE human skin tissue with Cdc37 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.

Flow cytometry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Flow cytometry testing of human MCF7 cells with Cdc37 antibody; Blue=isotype control, Green= Cdc37 antibody.