TA505394

antibody from OriGene

Targeting: NKIRAS1 kappaB-Ras1, KBRAS1

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

TA505394

Provider

OriGene

Proper citation

OriGene Cat#TA505394, RRID:AB_2623056

Product name

NKIRAS1 mouse monoclonal antibody, clone OTI6C11 (formerly 6C11)

Antibody type

Monoclonal

Description

NKIRAS1 mouse monoclonal antibody, clone OTI6C11 (formerly 6C11)

Host

Mouse

Conjugate

Unconjugated

Epitope

NKIRAS1

Isotype

IgG

Antibody clone number

OTI6C11

Vial size

100 µl

Concentration

1 mg/ml

Western blot

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NKIRAS1 (RC204248, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NKIRAS1.

Validation comment

WB

Immunocytochemistry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Anti-NKIRAS1 mouse monoclonal antibody (TA505394) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY NKIRAS1(RC204248).

Validation comment

IF

Immunohistochemistry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-NKIRAS1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA505394)

Validation comment

IHC

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Immunohistochemical staining of paraffin-embedded Human bladder tissue within the normal limits using anti-NKIRAS1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA505394)

Validation comment

IHC

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-NKIRAS1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA505394)

Validation comment

IHC