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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [1]
- Immunohistochemistry [1]
- Other assay [1]
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- Product number
- PA5-41072 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CATSPER2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Peptide sequence: HLQGLSQAVP RHTIRELLDP SRQKKLVLGD QHQLVRFSIK PQRIEQISHA Sequence homology: Cow: 85%; Horse: 92%; Human: 100%; Pig: 92%; Rabbit: 100%
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
Submitted references Low-density lipoproteins protect sperm during cryopreservation in buffalo: Unraveling mechanism of action.
Dalal J, Chandolia RK, Pawaria S, Kumar A, Kumar D, Selokar NL, Andonissamy J, Yadav PS, Kumar P
Molecular reproduction and development 2020 Dec;87(12):1231-1244
Molecular reproduction and development 2020 Dec;87(12):1231-1244
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of human Jurkat cell lysate using an anti-CATSPER2 polyclonal antibody (Product # PA5-41072).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of human brain cells using an anti-CATSPER2 polyclonal antibody (Product # PA5-41072).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- 5 Figure Evidence of CatSper channel functionality and intracellular calcium. (a) Western blot analysis of sperm lysates indicates a high protein level of CatSper-2 proteins in the sperm cryopreserved in LDL extenders. (b,c) A relative quantification of CatSper-1 and CatSper-2 in a cryopreserved sperm. (d) Intracellular calcium estimation by flow cytometry using Fluo-3 AM/PI. Sperm gating was performed using a logarithm of side and forward scatter. A total of 10,000 events were examined for each sample. The gated sperm divided was into four windows--lower left (a): live sperm with low intracellular calcium; lower right (b): live sperm with high intracellular calcium; upper left (c): dead sperm with low intracellular calcium; upper right (d): dead sperm with high intracellular calcium. (e) The percentage of the live sperm with low intracellular calcium, live sperm with high intracellular calcium, dead sperm with low intracellular calcium, and dead sperm with high intracellular calcium. Values (mean +- SE ) with different letters differ significantly ( p < .05), n = 20. EY, egg yolk; LDL, low-density lipoprotein
