PA5-41072

antibody from Invitrogen Antibodies

Targeting: CATSPER2

Western blot (Data presented on provider website)

Immunohistochemistry (Data presented on provider website)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-41072

Provider

Invitrogen Antibodies

Product name

CATSPER2 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

Peptide sequence: HLQGLSQAVP RHTIRELLDP SRQKKLVLGD QHQLVRFSIK PQRIEQISHA Sequence homology: Cow: 85%; Horse: 92%; Human: 100%; Pig: 92%; Rabbit: 100%

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

1 mg/mL

Storage

-20° C, Avoid Freeze/Thaw Cycles

References

Submitted references

Low-density lipoproteins protect sperm during cryopreservation in buffalo: Unraveling mechanism of action.

Dalal J, Chandolia RK, Pawaria S, Kumar A, Kumar D, Selokar NL, Andonissamy J, Yadav PS, Kumar P
Molecular reproduction and development 2020 Dec;87(12):1231-1244

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

5 Figure Evidence of CatSper channel functionality and intracellular calcium. (a) Western blot analysis of sperm lysates indicates a high protein level of CatSper-2 proteins in the sperm cryopreserved in LDL extenders. (b,c) A relative quantification of CatSper-1 and CatSper-2 in a cryopreserved sperm. (d) Intracellular calcium estimation by flow cytometry using Fluo-3 AM/PI. Sperm gating was performed using a logarithm of side and forward scatter. A total of 10,000 events were examined for each sample. The gated sperm divided was into four windows--lower left (a): live sperm with low intracellular calcium; lower right (b): live sperm with high intracellular calcium; upper left (c): dead sperm with low intracellular calcium; upper right (d): dead sperm with high intracellular calcium. (e) The percentage of the live sperm with low intracellular calcium, live sperm with high intracellular calcium, dead sperm with low intracellular calcium, and dead sperm with high intracellular calcium. Values (mean +- SE ) with different letters differ significantly ( p < .05), n = 20. EY, egg yolk; LDL, low-density lipoprotein