GTX130355

antibody from GeneTex

Targeting: UBE2I UBC9

Western blot (Supportive data in Antibodypedia)

Immunoprecipitation (Supportive data in Antibodypedia)

Immunohistochemistry (Data presented on provider website)

Antibody Data

Product number

GTX130355

Provider

GeneTex

Product name

UBE2I antibody

Antibody type

Polyclonal

Reactivity

Human, Mouse

Host

Rabbit

Western blot

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

UBC9 antibody detects UBC9 protein by western blot analysis. Various whole cell extracts (30 £gg) were separated by 15% SDS-PAGE, and the membrane was blotted with UBC9 antibody (GTX130355) diluted by 1:1000.

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

UBC9 antibody detects UBC9 protein by western blot analysis. Mouse tissue extracts (50 £gg) was separated by 15 % SDS-PAGE, and the membrane was blotted with UBC9 antibody (GTX130355) diluted by 1:1600.

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Various whole cell extracts (30 ?g) were separated by 15% SDS-PAGE, and the membranes were blotted with UBE2I antibody (GTX130355) diluted at 1:1000 and competitor's antibody (SC-10759) diluted by 1:500.

Immunoprecipitation

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Immunoprecipitation of UBE2I protein from 293T whole cell extracts using 5 £gg of UBE2I antibody (GTX130355).Western blot analysis was performed using UBE2I antibody (GTX130355).EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.