- PA5-78212 - Invitrogen Antibodies UBE2I antibody

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Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: Western blot analysis of UBE2I in whole cell lysate using 30 µg of protein. Samples were separated with 15% SDS-PAGE and incubated with UBE2I polyclonal antibody (Product # PA5-78212) using a dilution of 1:1000.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of UBE2I in mouse tissue using 50 µg of protein. Samples were separated with 15 % SDS-PAGE and incubated with UBE2I polyclonal antibody (Product # PA5-78212) using a dilution of 1:1600.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of UBE2I in whole cell lysate using 30 µg of protein. Samples were separated with 15% SDS-PAGE and incubated with UBE2I polyclonal antibody (Product # PA5-78212) using a dilution of 1:1000.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: UBC9 antibody detects UBC9 protein by western blot analysis. Mouse tissue extracts (50 µg) was separated by 15 % SDS-PAGE, and the membrane was blotted with UBC9 antibody UBC9 Polyclonal Antibody (Product # PA5-78212) diluted by 1:1600.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: UBC9 antibody detects UBC9 protein by western blot analysis. Various whole cell extracts (30 µg) were separated by 15% SDS-PAGE, and the membrane was blotted with UBC9 antibody UBC9 Polyclonal Antibody (Product # PA5-78212) diluted by 1:1,000.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot was performed using Anti-UBC9 Polyclonal Antibody (Product # PA5-78212) and a 18 kDa band corresponding to UBC9 was observed across all the cell lines and tissues tested. Whole cell extracts (30 µg lysate) of HeLa (Lane 1), Hep-G2 (Lane 2), MCF7 (Lane 3), BJ (Lane 4), MDA-MB-231 (Lane 5), 3T3-L1 (Lane 6), 3T3-L1 differentiated to adipocytes (Lane 7), tissue extracts (30ug lysate) of Mouse Spleen (Lane 8), Rat Spleen (Lane 9), Mouse Thymus (Lane 10) and Mouse Heart (Lane 11) were electrophoresed using NuPAGE® 4-12 % Bis-Tris gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:2000 dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: Immunofluorescence analysis of UBC9 was performed using 70% confluent log phase MCF7 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with UBC9 Polyclonal Antibody (Product # PA5-78212) at 1 µg/mL in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing localization to the nucleus and cytoplasm. Panel e represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: Immunohistochemistry (Paraffin) analysis of UBC9 was performed in paraffin-embedded human tonsil tissue using UBC9 Polyclonal Antibody (Product # PA5-78212) at a dilution of 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

PA5-78212