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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- Ab133246 - Provider product page
- Provider
- Aladdin Scientific
- Product name
- Recombinant UBE2I Antibody
- Antibody type
- Recombinant
- Description
- Rabbit anti Human UBC9 Antibody, Recombinant, could be used for WB, IHC, ICC, IF and so on.Application:WB: 1/500-1/1000ICC/IF: 1/50-1/200IHC: 1/50-1/100Protein FunctionAccepts the ubiquitin-like proteins SUMO1, SUMO2, SUMO3 and SUMO4 from the UBLE1A-UBLE1B E1 complex and catalyzes their covalent attachment to other proteins with the help of an E3 ligase such as RANBP2 or CBX4. Necessary for sumoylation of FOXL2 and KAT5. Essential for nuclear architecture and chromosome segregation.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Conjugate
- Unconjugated
- Antigen sequence
AA 28-40- Vial size
- 100_l,10_l,1ml,50_l
- Concentration
- 0,5 mg/ml
- Storage
- Store at 4¡C short term (1-2 weeks). Store at -20¡C long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle.
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Supportive validation
- Submitted by
- Aladdin Scientific (provider)
- Main image
- Experimental details
- Recombinant UBE2I Antibody (Ab133246) - Western Blot All lanes: Recombinant UBE2I Antibody (Ab133246) at 1/4000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution Predicted band size: 18 kDa Observed band size: 18 kDa Exposure time: 7.7 s
Supportive validation
- Submitted by
- Aladdin Scientific (provider)
- Main image
- Experimental details
- Recombinant UBE2I Antibody (Ab133246) - IF/ICC Immunocytochemistry analysis of SUMO Conjugating Enzyme UBC9 (green) in Hela using Recombinant UBE2I Antibody (Ab133246), and cells were counterstained with DAPI (blue).
Supportive validation
- Submitted by
- Aladdin Scientific (provider)
- Main image
- Experimental details
- Recombinant UBE2I Antibody (Ab133246) - IHC Immunohistochemistry analysis of paraffin-embedded Human tonsil using Recombinant UBE2I Antibody (Ab133246). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
