PA1-9111

antibody from Invitrogen Antibodies

Targeting: UBE2I UBC9

Antibody data

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Validation data

UBE2I protein structure - PA1-9111 shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of UBC9 by a UBC9 monoclonal antibody (Product # PA1-9111) at a concentration of 0.1 µg/mL. Human Kidney lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of UBC9 by a UBC9 monoclonal antibody (Product # PA1-9111) at a concentration of 0.3 µg/mL. Rat Ovary lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot was performed using Anti-UBC9 Polyclonal Antibody (Product # PA1-9111) and a 18 kDa band corresponding to UBC9 was observed across all the cell lines and tissues tested. Whole cell extracts (30 µg lysate) of HeLa (Lane 1), Hep G2 (Lane 2), MCF7 (Lane 3), BJ (Lane 4), MDA-MB-231 (Lane 5), 3T3-L1 (Lane 6), 3T3-L1 differentiated to adipocytes (Lane 7), tissue extracts (30ug lysate) of Mouse Spleen (Lane 8), Rat Spleen (Lane 9), Mouse Thymus (Lane 10) and Mouse Heart (Lane 11) were electrophoresed using NuPAGE® 4-12 % Bis-Tris gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:2500 dilution) and detected by chemiluminescence with Rabbit anti-Goat IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27014, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).