Antibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- PAB3478 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB3478, RRID:AB_1571316
- Product name
- ARAF polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against synthetic peptide of ARAF.
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references Selective activation of MEK1 but not MEK2 by A-Raf from epidermal growth factor-stimulated Hela cells.
The complete sequence and promoter activity of the human A-raf-1 gene (ARAF1).
Localization of the pKs gene, a raf related sequence on human chromosomes X and 7.
Wu X, Noh SJ, Zhou G, Dixon JE, Guan KL
The Journal of biological chemistry 1996 Feb 9;271(6):3265-71
The Journal of biological chemistry 1996 Feb 9;271(6):3265-71
The complete sequence and promoter activity of the human A-raf-1 gene (ARAF1).
Lee JE, Beck TW, Brennscheidt U, DeGennaro LJ, Rapp UR
Genomics 1994 Mar 1;20(1):43-55
Genomics 1994 Mar 1;20(1):43-55
Localization of the pKs gene, a raf related sequence on human chromosomes X and 7.
Popescu NC, Mark GE
Oncogene 1989 Apr;4(4):517-9
Oncogene 1989 Apr;4(4):517-9
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot analysis of MCF-7 cell lysate (35 ug/lane) with ARAF polyclonal antibody (Cat # PAB3478).
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Formalin-fixed and paraffin-embedded human cancer tissue reacted with ARAF polyclonal antibody (Cat # PAB3478) , which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated. BC = breast carcinoma.
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)