H00000369-D01P

antibody from Abnova Corporation

Targeting: ARAF ARAF1

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Proximity ligation assay (Supportive data in Antibodypedia)

Antibody Data

Product number

H00000369-D01P

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#H00000369-D01P, RRID:AB_1672987

Product name

ARAF purified MaxPab rabbit polyclonal antibody (D01P)

Antibody type

Polyclonal

Description

Rabbit polyclonal antibody raised against a full-length human ARAF protein.

Antigen sequence

MEPPRGPPANGAEPSRAVGTVKVYLPNKQRTVVTV
RDGMSVYDSLDKALKVRGLNQDCCVVYRLIKGRKT
VTAWDTAIAPLDGEELIVEVLEDVPLTMHNFVRKT
FFSLAFCDFCLKFLFHGFRCQTCGYKFHQHCSSKV
PTVCVDMSTNRQQFYHSVQDLSGGSRQHEAPSNRP
LNELLTPQGPSPRTQHCDPEHFPFPAPANAPLQRI
RSTSTPNVHMVSTTAPMDSNLIQLTGQSFSTDAAG
SRGGSDGTPRGSPSPASVSSGRKSPHSKSPAEQRE
RKSLADDKKKVKNLGYRDSGYYWEVPPSEVQLLKR
IGTGSFGTVFRGRWHGDVAVKVLKVSQPTAEQAQA
FKNEMQVLRKTRHVNILLFMGFMTRPGFAIITQWC
EGSSLYHHLHVADTRFDMVQLIDVARQTAQGMDYL
HAKNIIHRDLKSNNIFLHEGLTVKIGDFGLATVKT
RWSGAQPLEQPSGSVLWMAAEVIRMQDPNPYSFQS
DVYAYGVVLYELMTGSLPYSHIGCRDQIIFMVGRG
YLSPDLSKISSNCPKAMRRLLSDCLKFQREERPLF
PQILATIELLQRSLPKIERSASEPSLHRTQADELP
ACLLSAARLVP


Storage

Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

ARAF MaxPab rabbit polyclonal antibody. Western Blot analysis of ARAF expression in human placenta.

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western Blot analysis of ARAF expression in transfected 293T cell line (H00000369-T03) by ARAF MaxPab polyclonal antibody.Lane 1: ARAF transfected lysate(67.60 KDa).Lane 2: Non-transfected lysate.

Immunocytochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunofluorescence of purified MaxPab antibody to ARAF on HeLa cell. [antibody concentration 30 ug/ml]

Validation comment

Immunofluorescence

Protocol

Protocol

Proximity ligation assay

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Proximity Ligation Analysis of protein-protein interactions between ARAF and BRAF. HeLa cells were stained with anti-ARAF rabbit purified polyclonal 1:1200 and anti-BRAF mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).

Validation comment

In situ Proximity Ligation Assay (Cell)