H00000369-M05

antibody from Novus Biologicals

Targeting: ARAF ARAF1

Western blot (Supportive data in Antibodypedia)

ELISA (Supportive data in Antibodypedia)

Proximity ligation assay (Data presented on provider website)

Antibody Data

Product number

H00000369-M05

Provider

Novus Biologicals

Proper citation

Novus Cat#H00000369-M05, RRID:AB_2060499

Product name

Mouse Monoclonal A-RAF Antibody

Antibody type

Monoclonal

Description

IgG purified. ARAF - v-raf murine sarcoma 3611 viral oncogene homolog

Reactivity

Human

Host

Mouse

Isotype

IgG

Vial size

0.1 mg

Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: A-RAF Antibody (3E2) [H00000369-M05] - Analysis of ARAF expression in transfected 293T cell line by ARAF monoclonal antibody (M05), clone 3E2. Lane 1: ARAF transfected lysatE (67.6 KDa). Lane 2: Non-transfected lysate.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: A-RAF Antibody (3E2) [H00000369-M05] - Analysis of ARAF expression in HeLa (Cat # L013V1).

ELISA

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

ELISA: A-RAF Antibody (3E2) [H00000369-M05] - Detection limit for recombinant GST tagged ARAF is approximately 0.1ng/ml as a capture antibody.