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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunocytochemistry [2]
- Immunohistochemistry [1]
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- Product number
- PA5-21354 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- A-Raf Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: 293T, A431, H1299, HeLa, HepG2, Molt-4, Raji, NIH-3T3.
- Concentration
- 0.85 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using A-Raf Polyclonal Antibody (Product # PA5-21354). Sample (30 µg of whole cell lysate). Lane A: Hela . 7.5% SDS PAGE. A-Raf Polyclonal Antibody (Product # PA5-21354) diluted at 1:1,000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using A-Raf Polyclonal Antibody (Product # PA5-21354). Sample (30 µg of whole cell lysate). A: NIH-3T3. 7.5% SDS PAGE. A-Raf Polyclonal Antibody (Product # PA5-21354) diluted at 1:1,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of ARAF in paraformaldehyde-fixed A431 cells using an ARAF polyclonal antibody (Product # PA5-21354) at a 1:200 dilution.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of paraformaldehyde-fixed A431, using ARAF (Product # PA5-21354) antibody at 1:200 dilution.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using ARAF (Product # PA5-21354) antibody at 1:500 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
