PA5-13225

antibody from Invitrogen Antibodies

Targeting: SIRT6

Western blot (Data presented on provider website)

Immunohistochemistry (Data presented on provider website)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-13225

Provider

Invitrogen Antibodies

Product name

SIRT6 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Reactivity

Human, Mouse

Host

Rabbit

Isotype

IgG

Vial size

400 µL

Concentration

0.5 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

References

Submitted references

Low Rap1-interacting factor 1 and sirtuin 6 expression predict poor outcome in radiotherapy-treated Hodgkin lymphoma patients.

Bur H, Haapasaari KM, Turpeenniemi-Hujanen T, Kuittinen O, Auvinen P, Marin K, Soini Y, Karihtala P
Leukemia & lymphoma 2018 Mar;59(3):679-689

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Figure 1. Immunostaining of SIRT1, SIRT6, MGMT, and Rif1 expression in classical Hodgkin lymphoma. All figures are at x20 magnification. (A) Cytoplasmic SIRT1 expression was weak in Reed-Sternberg (RS) cells. In the cellular infiltrate, SIRT1 showed strong nuclear staining. (B) Very weak SIRT6 expression in the cytoplasm of RS cells. Expression in the cytoplasm of reactive cellular infiltrate was strong. (C) Very weak SIRT6 expression in the cytoplasm of RS cells; strong nuclear immunostaining in most of the reactive cellular infiltrate cells. (D) In RS cells, MGMT immunostaining was negative; strong nuclear immunostaining in most of the reactive cellular infiltrate cells. (E) Mostly very strong nuclear Rif1 expression in RS cells. Staining was partly granular. In the reactive cellular infiltrate, there was some nuclear expression. (F) Mainly very strong nuclear and weak to moderate cytoplasmic Rif1 expression in the RS cells. There was also some nuclear expression in the reactive cellular infiltrate.