Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [3]
- Immunohistochemistry [1]
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Validation data
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- Product number
- PA5-31359 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- DNAJB5 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: Jurkat, mouse brain.
- Concentration
- 1 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of DNAJB5 using 30 µg of Jurkat lysate. Samples were loaded onto a 10% SDS-PAGE gel and probed with a DNAJB5 polyclonal antibody (Product # PA5-31359) at a dilution of 1:500.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of DNAJB5 using 50 µg of mouse brain lysate. Samples were loaded onto a 10% SDS-PAGE gel and probed with a DNAJB5 polyclonal antibody (Product # PA5-31359) at a dilution of 1:500.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of DNAJB5 was performed by separating 30 µg of various whole cell extracts by 10% SDS-PAGE. Proteins were transferred to a membrane and probed with a DNAJB5 Polyclonal Antibody (Product # PA5-31359) at a dilution of 1:500 and a HRP-conjugated anti-rabbit IgG secondary antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- DNAJB5 Polyclonal Antibody detects DNAJB5 protein at cytosol on human colon carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded colon carcinoma. DNAJB5 Polyclonal Antibody (Product # PA5-31359) dilution: 1:200. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.