Explore
Validate
Learn
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [4]
Submit
Validation data
Reference
Comment
Report error
- Product number
- PA5-52060 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- RBM23 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: MASDDFDIVI EAMLEAPYKK EEDEQQRKEV KKDYPSNTTS STSNSGNETS GSSTIGETSN RSRDRDRYRR RNSRSRSPGR QCRHRSRSWD RRHGSESRSR DHRREDRVHY RSPPLATGYR YGHSKSPHFR EKSP Highest antigen sequence identity to the following orthologs: Mouse - 40%, Rat - 36%.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references Aryl Sulfonamides Induce Degradation of Aryl Hydrocarbon Receptor Nuclear Translocator through CRL4(DCAF15) E3 Ligase.
Structural basis of indisulam-mediated RBM39 recruitment to DCAF15 E3 ligase complex.
Kim SA, Jo SH, Cho JH, Yu MY, Shin HC, Kim JA, Park SG, Park BC, Kim S, Kim JH
Molecules and cells 2020 Nov 30;43(11):935-944
Molecules and cells 2020 Nov 30;43(11):935-944
Structural basis of indisulam-mediated RBM39 recruitment to DCAF15 E3 ligase complex.
Bussiere DE, Xie L, Srinivas H, Shu W, Burke A, Be C, Zhao J, Godbole A, King D, Karki RG, Hornak V, Xu F, Cobb J, Carte N, Frank AO, Frommlet A, Graff P, Knapp M, Fazal A, Okram B, Jiang S, Michellys PY, Beckwith R, Voshol H, Wiesmann C, Solomon JM, Paulk J
Nature chemical biology 2020 Jan;16(1):15-23
Nature chemical biology 2020 Jan;16(1):15-23
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of RBM23 in Lane 1: Marker (kDa) 230, 110, 82, 49, 32, 26, 18; Lane 2: Human cell line RT-4; Lane 3: Human cell line U-251MG sp. Samples were probed using a RBM23 Polyclonal Antibody (Product # PA5-52060).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of RBM23 in control (vector only transfected HEK293T lysate) and RBM23 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells). Samples were probed using a RBM23 Polyclonal Antibody (Product # PA5-52060).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent staining of RBM23 in human cell line U-2 OS shows positivity in intermediate filaments. Samples were probed using a RBM23 Polyclonal Antibody (Product # PA5-52060).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of RBM23 in human fallopian tube using a RBM23 Polyclonal Antibody (Product # PA5-52060) shows moderate cytoplasmic positivity in glandular cells.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of RBM23 in human testis using RBM23 Polyclonal Antibody (Product # PA5-52060) shows weak to moderate cytoplasmic positivity in cells in seminiferous ducts.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of RBM23 in human placenta using RBM23 Polyclonal Antibody (Product # PA5-52060) shows weak cytoplasmic positivity in trophoblastic cells.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of RBM23 in human fallopian tube using RBM23 Polyclonal Antibody (Product # PA5-52060) shows moderate cytoplasmic positivity in glandular cells.
