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Western blot
Other assayAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [3]
- Immunocytochemistry [2]
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- Product number
- PA5-21284 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- ERCC8 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: 293T, A431, Neuro2A, GL261, C8D30, NIH-3T3, Raw264.7, C2C12. Predicted reactivity: Mouse (93%), Rat (96%), Xenopus laevis (83%), Bovine (95%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of ERCC8 (CSA) using 30 µg of A) 293T and B) A431 lysate. Samples were loaded onto a 12% SDS-PAGE gel and probed with an ERCC8 (CSA) polyclonal antibody (Product # PA5-21284) at a dilution of 1:500.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using ERCC8 Polyclonal Antibody (Product # PA5-21284). Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with ERCC8 Polyclonal Antibody (Product # PA5-21284) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- ERCC8 Polyclonal Antibody detects ERCC8 protein by Western blot analysis. A. 30 µg Neuro2A whole cell lysate/extract. B. 30 µg GL261 whole cell lysate/extract. C. 30 µg C8D30 whole cell lysate/extract. D. 30 µg NIH-3T3 whole cell lysate/extract. E. 30 µg Raw264.7 whole cell lysate/extract. F. 30 µg C2C12 whole cell lysate/extract.10 % SDS-PAGE. ERCC8 Polyclonal Antibody (Product # PA5-21284) dilution: 1:1,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of ERCC8 (CSA) in paraformaldehyde-fixed A431 cells using an ERCC8 (CSA) polyclonal antibody (Product # PA5-21284) at a 1:200 dilution.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of paraformaldehyde-fixed A431, using ERCC8 (CSA) antibody (Product # PA5-21284) at 1:200 dilution.
