MS608PABX

antibody from Invitrogen Antibodies

Targeting: TIMP1 CLGI, EPO, TIMP

Antibody data

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Validation data

Product number: MS608PABX - Provider product page
Provider: Invitrogen Antibodies
Product name: TIMP1 Monoclonal Antibody (102D1)
Antibody type: Monoclonal
Antigen: Recombinant full-length protein
Description: MS608PABX targets TIMP-1 in immunohistochemistry and western blot applications and shows reactivity with human samples. The MS608PABX immunogen is recombinant human TIMP-1. MS608PABX detects TIMP-1 which has a predicted molecular weight of approximately 21 kDa.
Reactivity: Human
Host: Mouse
Isotype: IgG
Antibody clone number: 102D1
Vial size: 200 µg
Concentration: 1 mg/mL
Storage: -20°C

TIMP1 protein structure - MS608PABX shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis was performed on (Fig 1) whole cell extracts (30 µg lysate) of HeLa (Lane 1), HT-29 (Lane 2), SK-OV-3 (Lane 3), MCF7 (Lane 4), PC-3 (Lane 5), K-562 (Lane 6) and HCT 116 (Lane 7). Likewise Western blot analysis was performed on (Fig 2) 10 uL HeLa conditioned media (Lane 1) and 10 uL MDA-MB-231 conditioned media (Lane 2). The blots were probed with Anti-TIMP-1 Receptor Mouse Monoclonal Antibody (Product # MS608PABX, 1:500 dilution) and detected by chemiluminescence using Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A28177, 0.4 µg/mL, 1:2500 dilution). A 23 kDa band corresponding to TIMP-1 was observed across the cell lines and conditioned media tested. Known quantity of protein samples were electrophoresed using Novex® NuPAGE® 12 % Bis-Tris gel (Product # NP0342BOX), XCell SureLock™ Electrophoresis System (Product # EI0002) and Novex® Sharp Pre-Stained Protein Standard (Product # LC5800). Resolved proteins were then transferred onto a nitrocellulose membrane with iBlot® 2 Dry Blotting System (Product # IB21001). The membrane was probed with the relevant primary and secondary Antibody using iBind™ Flex Western Starter Kit (Product # SLF2000S). Chemiluminescent detection was performed using Pierce™ ECL Western Blotting Substrate (Product # 32106).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot was performed using Anti-TIMP1 Monoclonal Antibody (102D1) (Product # MS608PABX) and a 28kDa band corresponding to TIMP1 was observed in MDA-MB-231 but was absent in Daudi which is reported to be negative. An additional band at ~40kDa was observed in Daudi. Whole cell extracts (30 µg lysate) of MDA-MB-231 (Lane 1), MDA-MB-231 treated with Protein Transport Inhibitor (PTI; 1x for 4 hours) (Lane 2), Daudi (Lane 3) and Daudi treated with Protein Transport Inhibitor (1x for 4 hours) (Lane 4) were electrophoresed using Novex® NuPAGE® 4-12 % Bis-Tris gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:500 dilution) and detected by chemiluminescence with Goat anti-Mouse IgG (H+L), Superclonal™ Recombinant Secondary Antibody, HRP (Product # A28177, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).