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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [1]
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Validation data
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- Product number
- PA1-85923 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- TIMP1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- This antibody does not neutralize the protein activity.
- Concentration
- 1 mg/mL
Submitted references Effect of prostaglandin analogues on tear proteomics and expression of cytokines and matrix metalloproteinases in the conjunctiva and cornea.
Lopilly Park HY, Kim JH, Lee KM, Park CK
Experimental eye research 2012 Jan;94(1):13-21
Experimental eye research 2012 Jan;94(1):13-21
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Supportive validation
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- Invitrogen Antibodies (provider)
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- Experimental details
- Western blot was performed using Anti-TIMP1 Polyclonal Antibody (Product # PA1-85923) and a 28 kDa band corresponding to TIMP1 was observed in MDA-MB-231 treated with protein transport inhibitor in comparison to Daudi treated with protein transport inhibitor which is reported to be negative. Whole cell extracts (30 µg lysate) of MDA-MB-231 (Lane 1), MDA-MB-231 treated with PTI for 4 hr (Lane 2), Daudi (Lane 3), Daudi treated with PTI for 4 hr (Lane 4) were electrophoresed using Novex® NuPAGE® 4-12 % Bis-Tris gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody 5 µg/mL) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).
