ABIN2509280

antibody from antibodies-online

Targeting: TIMP1 CLGI, EPO, TIMP

ELISA (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

ABIN2509280

Provider

antibodies-online

Product name

anti-TIMP Metallopeptidase Inhibitor 1 (TIMP1) antibody

Antibody type

Polyclonal

Antigen

Other

Description

Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hTIMP-1 (human Tissue Inhibitor of Metalloproteinases-1). Anti-Human TIMP-1 specific antibody was purified by affinity chromatography employing immobilized hTIMP-1 matrix.

Reactivity

Human

Host

Rabbit

Vial size

100 μg

Storage

-20°C

ELISA

Supportive validation

Submitted by

antibodies-online (provider)

Main image

Experimental details

proNGF_(Mut-human) - proNGF (Mut-human) promotes neurite outgrowth in PC12 cells.Cells were grown on collagen-coated 24 well plates in DMEM medium supplemented with 6% fetal calf serum and 6% horse serum for 24 h. proNGF (Mut-human) (ABIN2515486) was added at the indicated concentration. The culture media was changed every 2-3 days. The development of neurites over a period of 5 days was visualized by Methylene Blue staining. proNGF_(Mut-human) - proNGF (Mut-human) does not promote survival of PC12 cells.Cells were grown in the absence of serum and collagen, and in the presence of varying concentrations of proNGF (Mut-human) (ABIN2515486). Cell survival relative to the control was measured by the XTT method, and plotted against proNGF (Mut-human) concentration.

Immunohistochemistry

Supportive validation

Submitted by

antibodies-online (provider)

Main image

Experimental details

Image(s): Immunohistochemistry