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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- ELISA [1]
- Immunoprecipitation [1]
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- Product number
- H00007076-M01 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#H00007076-M01, RRID:AB_548680
- Product name
- Mouse Monoclonal TIMP-1 Antibody
- Antibody type
- Monoclonal
- Description
- IgG purified. TIMP1 - TIMP metallopeptidase inhibitor 1
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: TIMP-1 Antibody (4D12) [H00007076-M01] - Analysis of TIMP1 expression in transfected 293T cell line by TIMP1 monoclonal antibody (M01), clone 4D12.Lane 1: TIMP1 transfected lysate(23.2 KDa).Lane 2: Non-transfected lysate.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- ELISA: TIMP-1 Antibody (4D12) [H00007076-M01] - Detection limit for recombinant GST tagged TIMP1 is approximately 3ng/ml as a capture antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: TIMP-1 Antibody (4D12) [H00007076-M01] - Analysis of TIMP1 transfected lysate using anti-TIMP1 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with TIMP1 MaxPab rabbit polyclonal antibody.
