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- References [4]
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- Product number
- A00561-2 - Provider product page
- Provider
- Boster Biological Technology
- Product name
- Anti-TIMP1 Antibody Picoband™
- Antibody type
- Polyclonal
- Description
- Rabbit IgG polyclonal antibody for TIMP1 detection. Tested with WB, FCM, Direct ELISA in Mouse;Rat.
- Reactivity
- Mouse, Rat
- Host
- Rabbit
- Vial size
- 100μg/vial
- Concentration
- Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
- Storage
- At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.
- Handling
- Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Submitted references Timosaponin BII inhibits TGF-β mediated epithelial-mesenchymal transition through Smad-dependent pathway during pulmonary fibrosis.
Adenovirus-mediated transfer of siRNA against PAI-1 mRNA ameliorates hepatic fibrosis in rats.
Effect of anluohuaxian tablet combined with gamma-IFN on schistosomal liver fibrosis.
Effects of 17 beta-estradiol on the expression of interstitial collagenases-8 and -13 (MMP-8 and MMP-13) and tissue inhibitor of metalloproteinase-1 (TIMP-1) in ovariectomized rat osteoblastic cells.
Ding D, Shen X, Yu L, Zheng Y, Liu Y, Wang W, Liu L, Zhao Z, Nian S, Liu L
Phytotherapy research : PTR 2023 Jul;37(7):2787-2799
Phytotherapy research : PTR 2023 Jul;37(7):2787-2799
Adenovirus-mediated transfer of siRNA against PAI-1 mRNA ameliorates hepatic fibrosis in rats.
Hu PF, Chen H, Zhong W, Lin Y, Zhang X, Chen YX, Xie WF
Journal of hepatology 2009 Jul;51(1):102-13
Journal of hepatology 2009 Jul;51(1):102-13
Effect of anluohuaxian tablet combined with gamma-IFN on schistosomal liver fibrosis.
Huang J, Huang H, Jiao Y, Ai G, Huang T, Li L, Yu H, Ma K, Xiao F
Journal of Huazhong University of Science and Technology. Medical sciences = Hua zhong ke ji da xue xue bao. Yi xue Ying De wen ban = Huazhong keji daxue xuebao. Yixue Yingdewen ban 2009 Feb;29(1):53-8
Journal of Huazhong University of Science and Technology. Medical sciences = Hua zhong ke ji da xue xue bao. Yi xue Ying De wen ban = Huazhong keji daxue xuebao. Yixue Yingdewen ban 2009 Feb;29(1):53-8
Effects of 17 beta-estradiol on the expression of interstitial collagenases-8 and -13 (MMP-8 and MMP-13) and tissue inhibitor of metalloproteinase-1 (TIMP-1) in ovariectomized rat osteoblastic cells.
Li J, Liao EY, Dai RC, Wei QY, Luo XH
Journal of molecular histology 2004 Nov;35(8-9):723-31
Journal of molecular histology 2004 Nov;35(8-9):723-31
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Supportive validation
- Submitted by
- Boster Biological Technology (provider)
- Main image
- Experimental details
- Western blot analysis of TIMP1 using anti-TIMP1 antibody (A00561-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions. Lane 1: mouse lung tissue lysates, Lane 2: mouse liver tissue lysates, Lane 3: mouse kidney tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TIMP1 antigen affinity purified polyclonal antibody (Catalog # A00561-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TIMP1 at approximately 28KD. The expected band size for TIMP1 is at 28KD.
- Additional image
