Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
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Validation data
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- Product number
- NB100-64749 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-64749, RRID:AB_963443
- Product name
- Mouse Monoclonal Properdin Antibody
- Antibody type
- Monoclonal
- Description
- Protein A purified. NB100-64749 recognizes human factor P, a 53 kDa glycosylated protein present in blood serum. Factor P binds to microbial surfaces and binds and stabilizes the complement component 3- and complement component 5-convertase enzyme complexes. C3b then acts as an opsonin or initiates the complement pathway again creating a positive feedback loop which acts only on the bacterial cell, leaving the host cells unharmed. Deficiency of factor P makes the host more susceptible to bacterial infections, particularly to Neisseria species and meningococcal infections.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.05 mg
- Concentration
- 1.0 mg/ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
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Supportive validation
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- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Properdin Antibody (10-18) [NB100-64749] - Human serum was run under reducing conditions on SDS PAGE and transferred to a PVDF membrane. In lane 1, Bio-Rad Precision Plus molecular weight marker was run. In lane 2, Mouse anti Human Factor P was used as the primary antibody. HRP conjugated Goat anti Mouse IgG (H/L) was used as the secondary antibody. As a control, HRP conjugated Goat anti Mouse IgG (H/L) was run in lane 3. Visualization was carried out using the Bio-Rad ChemiDoc Imaging System with automatic exposure.