LS-C289712

antibody from LSBio

Targeting: EIF3F eIF3-epsilon, eIF3-p47, EIF3S5

Western blot (Supportive data in Antibodypedia)

Immunoprecipitation (Recommended by provider)

Immunohistochemistry (Data presented on provider website)

Antibody Data

Product number

LS-C289712

Provider

LSBio

Product name

Anti-EIF3S5 / EIF3F Antibody (aa307-357) LS-C289712

Antibody type

Polyclonal

Description

Immunoaffinity purified

Reactivity

Human, Mouse, Rat, Bovine, Canine, Guinea Pig, Horse, Rabbit

Host

Rabbit

Isotype

IgG

Vial size

100µl

Storage

Store at 2-8°C for up to 1 year.

Western blot

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Detection of Human and Mouse eIF3F by Western Blot (h&m) and Immunoprecipitation (h). Samples: Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) and mouse NIH3T3 (M; 50 ug) cells. Antibodies: Affinity purified rabbit anti-eIF3F antibody used for WB at 0.04 ug/ml (A) and 0.4 ug/ml (B) and used for IP at 6 ug/mg lysate. eIF3F was also immunoprecipitated by rabbit anti-eIF3F antibody BL10962, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 30 seconds (B).