Explore
Validate
Learn
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- PAB10008 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB10008, RRID:AB_1674781
- Product name
- EIF3F polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against synthetic peptide of EIF3F.
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references Structure of cDNAs encoding human eukaryotic initiation factor 3 subunits. Possible roles in RNA binding and macromolecular assembly.
Asano K, Vornlocher HP, Richter-Cook NJ, Merrick WC, Hinnebusch AG, Hershey JW
The Journal of biological chemistry 1997 Oct 24;272(43):27042-52
The Journal of biological chemistry 1997 Oct 24;272(43):27042-52
No comments: Submit comment
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot using EIF3F polyclonal antibody (Cat # PAB10008) shows detection of endogenous EIF3F in lysates from both control HeLa cells (lane 1) and HeLa cells transformed with the kinase cdk11 (lane 2).Cdk11 is responsible for phosphorylating EIF3F in vivo.After SDS-PAGE and transfer, the membrane was probed with the primary antibody diluted to 1 : 200.This antibody recognizes both phosphorylated and non-phosphorylated eIF3f.Personal Communication, Jiaqi Shi, Univ. Arizona, Tucson, AZ.
