PA5-112249

antibody from Invitrogen Antibodies

Targeting: EIF3F eIF3-epsilon, eIF3-p47, EIF3S5

Western blot (Data presented on provider website)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Data presented on provider website)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-112249

Provider

Invitrogen Antibodies

Product name

eIF3f Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

1 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry-Immunofluorescence analysis of eIF3f in U2OS cells. Cells were fixed with 4% PFA, permeabilized with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with eIF3f Polyclonal Antibody (Product # PA5-112249) (1:200) at 4°C overnight. Then cells were stained with the Alexa Fluor 488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to Nucleus and cytoplasm.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunoprecipitation of eIF3f was performed on (Lane A) 0.5 mg HeLa whole cell lysate using 4 µL of eIF3f Polyclonal Antibody (Product # PA5-112249) at a dilution of 1:100, and 60 µg of Immunomagnetic beads Protein A/G. A Clean-Blot IP Detection Reagent (HRP) was used as a secondary antibody at a dilution of 1:1,000. Developed using the ECL technique and performed under reducing conditions. Predicted band size: 38 kDa. Observed band size: 45 kDa.