Explore
Validate
Learn
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
Submit
Validation data
Reference
Comment
Report error
- Product number
- PA5-27585 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- eIF3f Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: A431.
- Concentration
- 0.39 mg/mL
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of EIF3F using 30 µg of HeLa lysate. Samples were loaded onto a 10% SDS-PAGE gel and probed with an EIF3F polyclonal antibody (Product # PA5-27585) at a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of eIF3f was performed by separating 30 µg of various whole cell extracts by 10% SDS PAGE. Proteins were transferred to a membrane and probed with a eIF3f Polyclonal Antibody (Product # PA5-27585) at a dilution of 1:1000. A: A431, B: HepG2.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of EIF3F in methanol-fixed HeLa cells using an EIF3F polyclonal antibody (Product # PA5-27585) at a 1:200 dilution.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- eIF3f Polyclonal Antibody detects EIF3F protein at nucleus on mouse intestine by immunohistochemical analysis. Sample: Paraffin-embedded mouse intestine. EIF3f Polyclonal Antibody (Product # PA5-27585) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded BT483 xenograft, using EIF3F (Product # PA5-27585) antibody at 1:100 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
