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Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
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- Product number
- AP09323PU-N - Provider product page
- Provider
- Acris Antibodies GmbH
- Proper citation
- Acris Antibodies GmbH Cat#AP09323PU-N, RRID:AB_2035453
- Product name
- anti EIF3F / EIF3S5 (114-125)
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide corresponding to amino acids 114-125 of Human eIF3f protein.
- Reactivity
- Human, Mouse, Rat, Bovine, Chicken/Avian, Drosophila
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 mg
- Concentration
- 1.0 mg/ml (by UV absorbance at 280 nm)
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Supportive validation
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- Acris Antibodies GmbH (provider)
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- Experimental details
- Western blot using affinity purified AP09323PU-N eIF3f antibody shows detection of endogenous eIF3f in lysates from both control HeLa cells (Lane 1) and HeLa cells transformed with the kinase cdk11 (Lane 2). Cdk11 is responsible for phosphorylating eIF3f in vivo.After SDS-PAGE and transfer, the membrane was probed with the primary antibody diluted to 1/200. This antibody recognizes both phosphorylated and non-phosphorylated eIF3f.
