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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Flow cytometry [1]
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- Product number
- R32733 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- Tubby Antibody
- Antibody type
- Polyclonal
- Description
- This highly specific Tubby antibody is suitable for use in Western blot/Flow cytometry applications with human samples.
- Reactivity
- Human
- Host
- Rabbit
- Conjugate
- Unconjugated
- Vial size
- 100 ug
- Concentration
- 0.5mg/ml if reconstituted with 0.2ml sterile DI water
- Storage
- After reconstitution, the Tubby antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of human 1) COLO320 and 2) MCF7 cell lysate with Tubby antibody at 0.5ug/ml. Predicted molecular weight ~56/62 kDa (isoforms 1/2).
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Flow cytometry testing of human HeLa cells with Tubby antibody at 1ug/10^6 cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Tubby antibody.
