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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunocytochemistry [2]
- Immunohistochemistry [3]
- Other assay [1]
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- Product number
- PA5-31164 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- TUB Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: NT2D1, PC-3, U87-MG, SK-N-SH, Neuro2A, GL261.
- Concentration
- 1 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using TUB Polyclonal Antibody (Product # PA5-31164). Sample (30 µg of whole cell lysate). Lane A: Neuro2A. Lane B: GL261 . 7.5% SDS PAGE. TUB Polyclonal Antibody (Product # PA5-31164) diluted at 1:1,000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using TUB Polyclonal Antibody (Product # PA5-31164). Sample (30 µg of whole cell lysate). Lane A: NT2D1. Lane B: PC-3. Lane C: U87-MG. Lane D: SK-N-SH . 7.5% SDS PAGE. TUB Polyclonal Antibody (Product # PA5-31164) diluted at 1:1,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- TUB Polyclonal Antibody detects Tub protein at cytoplasm and nucleus by immunofluorescent analysis. Sample: SKNSH cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Tub protein stained by TUB Polyclonal Antibody (Product # PA5-31164) diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 µm.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- TUB Polyclonal Antibody detects Tub protein at cytoplasm and nucleus by immunofluorescent analysis. Sample: SKNSH cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Tub protein stained by TUB Polyclonal Antibody (Product # PA5-31164) diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 µm.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using Tub (Product # PA5-31164) antibody at 1:500 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- TUB Polyclonal Antibody detects Tub protein at nucleus on mouse fore brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse fore brain. TUB Polyclonal Antibody (Product # PA5-31164) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- TUB Polyclonal Antibody detects Tub protein at nucleus on rat fore brain by immunohistochemical analysis. Sample: Paraffin-embedded rat fore brain. TUB Polyclonal Antibody (Product # PA5-31164) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of Tub showing staining in the cytoplasm and nucleus of SKNSH cells. SKNSH cells were fixed in 4% paraformaldehyde at RT for 15 min and stained using a Tub polyclonal antibody (Product # PA5-31164) diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10µm.
