PA5-47974

antibody from Invitrogen Antibodies

Targeting: GDF3

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-47974

Provider

Invitrogen Antibodies

Product name

GDF3 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

In Western blots, less than 5% cross-reactivity with recombinant mouse (rm) GDF-5, rmGDF-6, rmGDF-7, rmGDF-8, and rmGDF-9 is observed.

Concentration

0.2 mg/mL

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis from lysates of BG01V human embryonic stem cells. PVDF membrane was probed with 1.5 µg/mL of Goat Anti-human/mouse GDF-3 Antigen Affinity-purified Polyclonal Antibody (Product # PA5-47974) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody. A specific band was detected for GDF-3 at approximately 42 kDa (as indicated). This experiment was conducted under reducing conditions.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of GDF3 in BG01V human embryonic stem cells. Samples were incubated in GDF3 polyclonal antibody (Product # PA5-47974) using a dilution of 1.5 µg/mL followed by a HRP-conjugated Anti-Goat IgG secondary antibody. A specific band was detected for GDF‚3 at approximately 42 kDa (as indicated). This experiment was conducted under reducing conditions.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry analysis of GDF3 in immersion fixed BG01V human embryonic stem cells. Samples were incubated in GDF3 polyclonal antibody (Product # PA5-47974) using a dilution of 10 µg/mL for 3 hours at room temperature followed by NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of GDF3 in perfusion fixed frozen sections of mouse thymus. Samples were incubated in GDF3 polyclonal antibody (Product # PA5-47974) using a dilution of 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of GDF3 in perfusion fixed frozen sections of mouse thymus. Samples were incubated in GDF3 polyclonal antibody (Product # PA5-47974) using a dilution of 5 µg/mL overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown) and counterstained with hematoxylin (blue).