Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
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Validation data
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- Product number
- PA5-47974 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- GDF3 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- In Western blots, less than 5% cross-reactivity with recombinant mouse (rm) GDF-5, rmGDF-6, rmGDF-7, rmGDF-8, and rmGDF-9 is observed. Reconstitute at 0.2 mg/mL in sterile PBS.
- Reactivity
- Human, Mouse
- Host
- Goat
- Isotype
- IgG
- Vial size
- 100 µg
- Concentration
- 0.2 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis from lysates of BG01V human embryonic stem cells. PVDF membrane was probed with 1.5 µg/mL of Goat Anti-human/mouse GDF-3 Antigen Affinity-purified Polyclonal Antibody (Product # PA5-47974) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody. A specific band was detected for GDF-3 at approximately 42 kDa (as indicated). This experiment was conducted under reducing conditions.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of GDF3 in BG01V human embryonic stem cells. Samples were incubated in GDF3 polyclonal antibody (Product # PA5-47974) using a dilution of 1.5 µg/mL followed by a HRP-conjugated Anti-Goat IgG secondary antibody. A specific band was detected for GDF‚3 at approximately 42 kDa (as indicated). This experiment was conducted under reducing conditions.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry analysis of GDF3 in immersion fixed BG01V human embryonic stem cells. Samples were incubated in GDF3 polyclonal antibody (Product # PA5-47974) using a dilution of 10 µg/mL for 3 hours at room temperature followed by NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of GDF3 in perfusion fixed frozen sections of mouse thymus. Samples were incubated in GDF3 polyclonal antibody (Product # PA5-47974) using a dilution of 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of GDF3 in perfusion fixed frozen sections of mouse thymus. Samples were incubated in GDF3 polyclonal antibody (Product # PA5-47974) using a dilution of 5 µg/mL overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown) and counterstained with hematoxylin (blue).