Antibody data

Product number
500-P235BT-1MG - Provider product page
Provider
Invitrogen Antibodies
Product name
GDF3 Polyclonal Antibody, Biotin, PeproTech®
Antibody type
Polyclonal
Antigen
Recombinant full-length protein
Description
AA Sequence of recombinant protein: AAIPVPKLSC KNLCHRHQLF INFRDLGWHK WIIAPKGFMA NYCHGECPFS LTISLNSSNY AFMQALMHAV DPEIPQAVCI PTKLSPISML YQDNNDNVIL RHYEDMVVDE CGCG. Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human GDF-3. Anti-Human GDF-3-specific antibody was purified by affinity chromatography and then biotinylated. Sandwich ELISA: To detect hGDF-3 by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.25-1.0 µg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Human GDF-3 (500-P235) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant hGDF-3. Western Blot: To detect hGDF-3 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant hGDF-3 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions. 500-P235BT-1MG will be provided as 2 x 500 µg
Reactivity
Human
Host
Rabbit
Conjugate
Biotin
Vial size
2 x 500 µg
Concentration
0.1-1.0 mg/mL
Storage
-20°C
GDF3 protein structure - 500-P235BT-1MG shown in red.