Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
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Validation data
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- Product number
- AF958 - Provider product page
- Provider
- R&D Systems
- Product name
- Human/Mouse GDF-3 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Detects human and mouse GDF-3 in direct ELISAs and Western blots. In Western blots, less than 5% cross-reactivity with recombinant mouse (rm) GDF-5, rmGDF-6, rmGDF-7, rmGDF-8, and rmGDF-9 is observed.
- Reactivity
- Human, Mouse
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
Q07104
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references GDF3 is a BMP inhibitor that can activate Nodal signaling only at very high doses.
Levine AJ, Levine ZJ, Brivanlou AH
Developmental biology 2009 Jan 1;325(1):43-8
Developmental biology 2009 Jan 1;325(1):43-8
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human GDF-3 by Western Blot. Western blot shows lysates of BG01V human embryonic stem cells. PVDF membrane was probed with 1.5 µg/mL of Goat Anti-Human/Mouse GDF-3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF958) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for GDF-3 at approximately 42 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- GDF-3 in BG01V Human Stem Cells. GDF-3 was detected in immersion fixed BG01V human embryonic stem cells using Goat Anti-Human/Mouse GDF-3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF958) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- GDF-3 in Mouse Thymus. GDF-3 was detected in perfusion fixed frozen sections of mouse thymus using 5 µg/mL Mouse GDF-3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF958) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- GDF-3 in Mouse Thymus. GDF-3 was detected in perfusion fixed frozen sections of mouse thymus using Mouse GDF-3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF958) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.