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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [3]
- Immunocytochemistry [2]
- Immunohistochemistry [1]
- Flow cytometry [2]
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- Product number
- CF501491 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- SH3GL1 Monoclonal Antibody (OTI2F5), TrueMAB™
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- OTI2F5
- Vial size
- 100 µg
- Concentration
- 1 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SH3GL1 (RC201552, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 µg per lane) were separated by SDS-PAGE and immunoblotted with anti-SH3GL. Positive lysates LY418946 (100 µg) and LC418946 (20 µg) can be purchased separately from OriGene.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of extracts (35 µg) from 9 different cell lines by using anti-SH3GL1 monoclonal antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Equivalent amounts of cell lysates (10 µg per lane) of wild-type 293T cells (WT, Cat# LC810293T) and SH3GL1-Knockout 293T cells (KO, Cat# LC812166) were separated by SDS-PAGE and immunoblotted with anti-SH3GL1 monoclonal antibody TA501491, (1:500). Then the blotted membrane was stripped and reprobed with anti-HSP90AB1 antibody (TA500494) as a loading control.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Anti-SH3GL1 mouse monoclonal antibody (TA501491) Immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY SH3GL1(RC201552).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Anti-SH3GL1 mouse monoclonal antibody (TA501491) Immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY SH3GL1(RC201552).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-SH3GL1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA501491)
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometric analysis of Hela cells, using anti-SH3GL1 antibody (TA501491), (Red), compared to a nonspecific negative control antibody (TA50011), (Blue).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometric analysis of Jurkat cells, using anti-SH3GL1 antibody (TA501491), (Red), compared to a nonspecific negative control antibody (TA50011), (Blue).
