Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- PAB21357 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB21357, RRID:AB_10968438
- Product name
- RNF216 polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against recombinant RNF216.
- Antigen sequence
KVIILEEGSLLYTESDPLETQNQSSEDSETELLSN
LGESAALADDQAIEEDCWLDHPYFQSLNQQPREIT
NQVVPQERQPEAELGRLLFQHEFPGPAFP- Isotype
- IgG
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunofluorescent staining of human cell line A-431 with RNF216 polyclonal antibody (Cat # PAB21357) at 1-4 ug/mL dilution shows positivity in nucleus but not nucleoli and cytoplasm.
- Validation comment
- Immunofluorescence
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunohistochemical staining of human testis with RNF216 polyclonal antibody (Cat # PAB21357) shows strong cytoplasmic positivity in developing sperm cells at 1:200-1:500 dilution.
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)