RQ7650

antibody from NSJ Bioreagents

Targeting: RNF216 TRIAD3, UBCE7IP1, ZIN

Western blot (Supportive data in Antibodypedia)

ELISA (Data presented on provider website)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

RQ7650

Provider

NSJ Bioreagents

Product name

RNF216 Antibody / TRIAD3

Antibody type

Polyclonal

Description

This highly specific RNF216 antibody is suitable for use in Western blot/Flow cytometry/Direct ELISA applications with human, mouse and rat samples.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Conjugate

Unconjugated

Vial size

100 ug

Concentration

0.5mg/ml if reconstituted with 0.2ml sterile DI water

Storage

After reconstitution, the RNF216 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Western blot testing of 1) human U-2 OS, 2) human MCF7, 3) human K562, 4) rat testis, 5) rat heart, 6) mouse testis and 7) mouse heart tissue lysate with RNF216 antibody. Predicted molecular weight ~99, ~106 and ~57 kDa (three isoforms).

Flow cytometry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Flow cytometry testing of human HepG2 cells with RNF216 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= RNF216 antibody.