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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [2]
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- Validations
- Western blot [2]
- Immunocytochemistry [2]
- Immunohistochemistry [1]
- Other assay [1]
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- Product number
- PA5-20079 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- RNF216 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- A suggested positive control is mouse heart tissue lysate.
- Concentration
- 1 mg/mL
Submitted references Interplay Between Sialic Acids, Siglec-E, and Neu1 Regulates MyD88- and TRIF-Dependent Pathways for TLR4-Activation During Leishmania donovani Infection.
Siglec-E Negatively Regulates the Activation of TLR4 by Controlling Its Endocytosis.
Karmakar J, Mandal C
Frontiers in immunology 2021;12:626110
Frontiers in immunology 2021;12:626110
Siglec-E Negatively Regulates the Activation of TLR4 by Controlling Its Endocytosis.
Wu Y, Ren D, Chen GY
Journal of immunology (Baltimore, Md. : 1950) 2016 Oct 15;197(8):3336-3347
Journal of immunology (Baltimore, Md. : 1950) 2016 Oct 15;197(8):3336-3347
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of mouse heart cell lysates using a TRIAD3A polyclonal antibody (Product # PA5-20079) at (A) 0.5, (B) 1, and (C) 2 µg/mL.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of TRIAD3A in mouse heart tissue lysate with RNF216 Polyclonal Antibody (Product # PA5-20079) at (A) 1 and (B) 2 µg/mL.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of mouse heart cells using a TRIAD3A polyclonal antibody (Product # PA5-20079) at a 20 µg/mL dilution.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence of TRIAD3A in Mouse Heart cells with RNF216 Polyclonal Antibody (Product # PA5-20079) at 20 µg/mL.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of TRIAD3A in mouse heart with RNF216 Polyclonal Antibody (Product # PA5-20079) at 10 µg/mL.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 5 Neu1 overexpression or siglec-E silencing prevented toll-like receptor 4 (TLR4) degradation. (A, B) Macrophages (untransfected or transfected) followed by infection were lysed. The lysates were immunoprecipitated with anti-TLR4 and further processed as mentioned in materials and methods. The blots were probed with anti-ubiquitin (A) and anti-Triad3a (B) . Data were obtained from three independent experiments and one representative blot is shown. Bar graphs represent band intensities. Significance: *p
