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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunoprecipitation [1]
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- Product number
- H00065975-M09 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#H00065975-M09, RRID:AB_566212
- Product name
- STK33 monoclonal antibody (M09), clone 1F10
- Antibody type
- Monoclonal
- Description
- Mouse monoclonal antibody raised against a full length recombinant STK33.
- Antigen sequence
MADSGLDKKSTKCPDCSSASQKDVLCVCSSKTRVP
PVLVVEMSQTSSIGSAESLISLERKKEKNINRDIT
SRKDLPSRTSNVERKASQQQWGRGNFTEGKVPHIR
IENGAAIEEIYTFGRILGKGSFGIVIEATDKETET
KWAIKKVNKEKAGSSAVKLLEREVNILKSVKHEHI
IHLEQVFETPKKMYLVMELCEDGELKEILDRKGHF
SENETRWIIQSLASAIAYLHNNDIVHRDLKLENIM
VKSSLIDDNNEINLNIKVTDFGLAVKKQSRSEAML
QATCGTPIYMAPEVISAHDYSQQCDIWSIGVVMYM
LLRGEPPFLASSEGKLFELIRKGELHFENAVWNSI
SDCAKSVLKQLMKVDPAHRITAKELLDNQWLTGNK
LSSVRPTNVLEMMKEWKNNPESVEENTTEEKNKPS
TEEKLKSYQPWGNVPETNYTSDEEEEKQSTTYEKQ
FPATSKDNFDMCSSSFTSSKLLPAEIKGEMEKTPV
TPSQGTATKYPAKSGALSRTKKKL- Isotype
- IgG
- Antibody clone number
- 1F10
- Storage
- Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
Submitted references STK33 kinase activity is nonessential in KRAS-dependent cancer cells.
Synthetic lethal interaction between oncogenic KRAS dependency and STK33 suppression in human cancer cells.
Babij C, Zhang Y, Kurzeja RJ, Munzli A, Shehabeldin A, Fernando M, Quon K, Kassner PD, Ruefli-Brasse AA, Watson VJ, Fajardo F, Jackson A, Zondlo J, Sun Y, Ellison AR, Plewa CA, San MT, Robinson J, McCarter J, Schwandner R, Judd T, Carnahan J, Dussault I
Cancer research 2011 Sep 1;71(17):5818-26
Cancer research 2011 Sep 1;71(17):5818-26
Synthetic lethal interaction between oncogenic KRAS dependency and STK33 suppression in human cancer cells.
Scholl C, Fröhling S, Dunn IF, Schinzel AC, Barbie DA, Kim SY, Silver SJ, Tamayo P, Wadlow RC, Ramaswamy S, Döhner K, Bullinger L, Sandy P, Boehm JS, Root DE, Jacks T, Hahn WC, Gilliland DG
Cell 2009 May 29;137(5):821-34
Cell 2009 May 29;137(5):821-34
No comments: Submit comment
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- STK33 monoclonal antibody (M09), clone 1F10. Western Blot analysis of STK33 expression in Raw 264.7(Cat # L024V1 ).
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western Blot analysis of STK33 expression in transfected 293T cell line by STK33 monoclonal antibody (M09), clone 1F10.Lane 1: STK33 transfected lysate(57.9 KDa).Lane 2: Non-transfected lysate.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunofluorescence of monoclonal antibody to STK33 on A-431 cell. [antibody concentration 10 ug/ml]
- Validation comment
- Immunofluorescence
- Protocol
- Protocol
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunoprecipitation of STK33 transfected lysate using anti-STK33 monoclonal antibody and Protein A Magnetic Bead (U0007), and immunoblotted with STK33 MaxPab rabbit polyclonal antibody.
- Validation comment
- Immunoprecipitation
- Protocol
- Protocol
