Antibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Western blot [3]
- Immunocytochemistry [1]
- Immunoprecipitation [1]
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Validation data
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- Product number
- H00065975-M09 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#H00065975-M09, RRID:AB_830250
- Product name
- Mouse Monoclonal STK33 Antibody
- Antibody type
- Monoclonal
- Description
- IgG purified. STK33 (1F10)
- Reactivity
- Human, Mouse, Rat
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Submitted references STK33 kinase activity is nonessential in KRAS-dependent cancer cells.
Synthetic lethal interaction between oncogenic KRAS dependency and STK33 suppression in human cancer cells.
Babij C, Zhang Y, Kurzeja RJ, Munzli A, Shehabeldin A, Fernando M, Quon K, Kassner PD, Ruefli-Brasse AA, Watson VJ, Fajardo F, Jackson A, Zondlo J, Sun Y, Ellison AR, Plewa CA, San MT, Robinson J, McCarter J, Schwandner R, Judd T, Carnahan J, Dussault I
Cancer research 2011 Sep 1;71(17):5818-26
Cancer research 2011 Sep 1;71(17):5818-26
Synthetic lethal interaction between oncogenic KRAS dependency and STK33 suppression in human cancer cells.
Scholl C, Fröhling S, Dunn IF, Schinzel AC, Barbie DA, Kim SY, Silver SJ, Tamayo P, Wadlow RC, Ramaswamy S, Döhner K, Bullinger L, Sandy P, Boehm JS, Root DE, Jacks T, Hahn WC, Gilliland DG
Cell 2009 May 29;137(5):821-34
Cell 2009 May 29;137(5):821-34
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: STK33 Antibody (1F10) [H00065975-M09] - Analysis of STK33 expression in transfected 293T cell line by STK33 monoclonal antibody (M09), clone 1F10. Lane 1: STK33 transfected lysatE (57.9 KDa). Lane 2: Non-transfected lysate.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: STK33 Antibody (1F10) [H00065975-M09] - Analysis of STK33 expression in PC-12 (Cat # L012V1).
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: STK33 Antibody (1F10) [H00065975-M09] - Analysis of STK33 expression in A-431 (Cat # L015V1).
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunocytochemistry/Immunofluorescence: STK33 Antibody (1F10) [H00065975-M09] - Analysis of monoclonal antibody to STK33 on A-431 cell. Antibody concentration 10 ug/ml
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: STK33 Antibody (1F10) [H00065975-M09] - Analysis of STK33 transfected lysate using anti-STK33 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with STK33 rabbit polyclonal antibody.