18436-1-AP

antibody from Proteintech Group

Targeting: PRMT5 HRMT1L5, SKB1, SKB1Hs

Western blot (Supportive data in Antibodypedia)

ELISA (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

18436-1-AP

Provider

Proteintech Group

Proper citation

Proteintech Cat#18436-1-AP, RRID:AB_2171798

Product name

PRMT5 antibody

Antibody type

Polyclonal

Description

KD/KO validated PRMT5 antibody (Cat. #18436-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse and has been validated for the following applications: IHC, WB,ELISA.

Reactivity

Human, Mouse

Host

Rabbit

Conjugate

Unconjugated

Isotype

IgG

Vial size

20ul, 150ul

References

Submitted references

A biomimetic arsenic-based nanozyme enhances dual epigenetic regulation to improve the efficacy of immunotherapy for acute myeloid leukaemia.

Chang A, Peng H, Chen Y, Zhang H, Xu X, Zhang K, Yang J, Li W, Wang X, Dong X, Ni J
Biomaterials 2026 Oct;333:124215

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DysUFMylation reprograms immunosuppressive neutrophils to potentiate anti-PD-1 therapy in hepatocellular carcinoma.

Gao X, Xu M, Han Z, Hu Z, Su Y, Sun G, Chen W, Qiu S, Qiu S, Hung MC, Zhou C, Yi Y
Cancer letters 2026 May 1;645:218395

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Inhibition of PRMT5 triggers synthetic lethality in ARID1A-deficient endometrial cancer by promoting aberrant R-loop accumulation.

Shu W, Dong K, Shen X, Zhou X, Zhang J, Yu S, Cheng S, Zhang T, Chen G, Zhong G, Zhang J, Wang H
Molecular cancer 2026 Mar 9;25(1)

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CUL5 E3 ubiquitin ligase regulates the evasion of bladder cancer cells to CD8+ T cell-mediated killing by inhibiting autophagy.

Gao X, Yu Y, Sun J, Zhao H, You Y, Shi X, Wang K, Hong S, Xiong X, Huang C, Zhang H, Jiang G
PLoS biology 2026 Feb;24(2):e3003647

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Dual Roles of SIRT7 Inhibition by Oroxylin A Reprogram HSCs Fate: PRMT5 Succinylation-Driven Senescence and Ecto-Calreticulin-Dependent NK Cell Immune Clearance in Liver Fibrosis.

Wang J, Tian H, Gao Y, Qiu X, Bao Z, Zhao D, Zhang F, Zhang Z, Wang F, Zheng S, Cheng H, Shao J
Research (Washington, D.C.) 2025;8:0808

---

PRMT5-Mediated Arginine Methylation of ACSL4 Attenuates Its Stability and Suppresses Ferroptosis in Renal Cancer.

Zhang M, Liu H, Yin R, Xu J, Fan S, Qian X, Cao M, Li S, Zhang A, Chen G, Yong H, Li Z, Bai J
Research (Washington, D.C.) 2025;8:0789

---

PRMT5 Maintains Homeostasis of the Intestinal Epithelium by Modulating Cell Proliferation and Survival.

Li L, Zhang Z, Wang X, Zhao H, Liu L, Xiao Y, Hua S, Chen YG
Advanced science (Weinheim, Baden-Wurttemberg, Germany) 2025 Mar;12(12):e2415559

---

PRMT5 Promotes Pancreatic Cancer Tumorigenesis via Positive PRMT5/C-Myc Feedback Loop.

Yang F, Song P, Xiao Z, Su R, Fang X, Wu Y, Xu X, Wang K
MedComm 2025 Jun;6(6):e70150

---

PRMT5 K240lac confers ferroptosis resistance via ALKBH5/SLC7A11 axis in colorectal cancer.

Qu S, Feng B, Xing M, Qiu Y, Ma L, Yang Z, Ji Y, Huang F, Wang Y, Zhou J, Xu M, He J, Zhou Q, Zhou X, Xiong W, Yao B, Liu M, Dong Q, Yang L, Gu S
Oncogene 2025 Aug;44(32):2814-2830

---

LncRNA EP300-AS1 interacts with PTBP1 to destabilize PRMT5 mRNA and suppresses NSCLC growth and metastasis.

Chen J, Fan C, Song S, Zhang D
Cell death & disease 2025 Aug 11;16(1):607

---

PRMT5 Inhibition Enhances Therapeutic Efficacy of Cisplatin via Mediating miR-29b-3p-Mcl-1 Expression in Lung Adenocarcinoma.

Li H, Fan J, Shen W, Zhang Y, Zhu X, Li P, Gu Z, Jing P
Cell biology international 2025 Apr;49(4):407-418

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Poly-GR Impairs PRMT1-Mediated Arginine Methylation of Disease-Linked RNA-Binding Proteins by Acting as a Substrate Sink.

Hutten S, Chen JX, Isaacs AM, Dormann D
Biochemistry 2024 Sep 3;63(17):2141-2152

---

Deciphering alternative splicing events and their therapeutic implications in colorectal Cancer.

Ding W, Xiao Q, Yue Y, Chen S, She X, Pan B, Zhou L, Yin Y, Li Y, Wang S, Xu M
Cellular signalling 2024 Jun;118:111134

---

PRMT5-mediated arginine methylation of FXR1 is essential for RNA binding in cancer cells.

Vijayakumar A, Majumder M, Yin S, Brobbey C, Karam J, Howley B, Howe PH, Berto S, Madan LK, Gan W, Palanisamy V
Nucleic acids research 2024 Jul 8;52(12):7225-7244

---

Protein arginine methyltransferase 5 confers the resistance of triple-negative breast cancer to nanoparticle albumin-bound paclitaxel by enhancing autophagy through the dimethylation of ULK1.

Kong J, Dong Y, Li M, Fan J, Wang T
Toxicology and applied pharmacology 2024 Dec;493:117145

---

Inhibitory Effect of PRMT5/MTA Inhibitor on MTAP-Deficient Glioma May Be Influenced by Surrounding Normal Cells.

Wang Y, Sun X, Ma R, Zhang X, Ji S, Liu Z, Yang G, Wang H, Zhang P, Zhang J, Tian J
Cancer medicine 2024 Dec;13(24):e70526

---

TBL2 Promotes Tumorigenesis via PRMT5/WDR77-Mediated AKT Activation in Breast Cancer.

Lu X, Zhang C, Zhu L, Wang S, Zeng L, Zhong W, Wu X, Yuan Q, Tang H, Cui S, Tan Y, Li Y, Wei W
Advanced science (Weinheim, Baden-Wurttemberg, Germany) 2024 Dec;11(47):e2400160

---

The PRMT5 inhibitor C9 mitigates hypoxia-induced carboplatin resistance in lung cancer by inducing autophagy.

Fan J, Li H, Ruan Q, Zhu X, Jing P, Gu Z
Cell biology international 2023 Oct;47(10):1702-1715

---

PRMT5 mediates FoxO1 methylation and subcellular localization to regulate lipophagy in myogenic progenitors.

Kim KH, Oprescu SN, Snyder MM, Kim A, Jia Z, Yue F, Kuang S
Cell reports 2023 Nov 28;42(11):113329

---

GSK3326595 is a promising drug to prevent SARS-CoV-2 Omicron and other variants infection by inhibiting ACE2-R671 di-methylation.

Li Z, Yong H, Wang W, Gao Y, Wang P, Chen X, Lu J, Zheng J, Bai J
Journal of medical virology 2023 Jan;95(1):e28158

---

PRMT5-mediated regulatory arginine methylation of RIPK3.

Chauhan C, Martinez-Val A, Niedenthal R, Olsen JV, Kotlyarov A, Bekker-Jensen S, Gaestel M, Menon MB
Cell death discovery 2023 Jan 19;9(1):14

---

MST2 methylation by PRMT5 inhibits Hippo signaling and promotes pancreatic cancer progression.

Sun Y, Jin X, Meng J, Guo F, Chen T, Zhao X, Wu H, Ren D
The EMBO journal 2023 Dec 1;42(23):e114558

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PRMT5 confers lipid metabolism reprogramming, tumour growth and metastasis depending on the SIRT7-mediated desuccinylation of PRMT5 K387 in tumours.

Yuan HF, Zhao M, Zhao LN, Yun HL, Yang G, Geng Y, Wang YF, Zheng W, Yuan Y, Song TQ, Niu JQ, Zhang XD
Acta pharmacologica Sinica 2022 Sep;43(9):2373-2385

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Discovery of novel PRMT5 inhibitors bearing a methylpiperazinyl moiety.

Bai X, Zhai Z, Zhao X, Li R, Liang L, Jin Y, Yin Y
Future medicinal chemistry 2022 Jul;14(14):1071-1086

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ZEB2/TWIST1/PRMT5/NuRD Multicomplex Contributes to the Epigenetic Regulation of EMT and Metastasis in Colorectal Carcinoma.

Zheng Y, Dai M, Dong Y, Yu H, Liu T, Feng X, Yu B, Zhang H, Wu J, Kong W, Yu X, Wu H
Cancers 2022 Jul 14;14(14)

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MYBL1 induces transcriptional activation of ANGPT2 to promote tumor angiogenesis and confer sorafenib resistance in human hepatocellular carcinoma.

Zhu J, Wu Y, Yu Y, Li Y, Shen J, Zhang R
Cell death & disease 2022 Aug 20;13(8):727

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Protein arginine methyltransferase 5 mediates THP-1-derived macrophage activation dependent on NF-κB in endometriosis.

Chai X, Wu X, He L, Ding H
Experimental and therapeutic medicine 2021 Sep;22(3):1003

---

Arginine methyltransferase PRMT5 negatively regulates cGAS-mediated antiviral immune response.

Ma D, Yang M, Wang Q, Sun C, Shi H, Jing W, Bi Y, Shen X, Ma X, Qin Z, Lin Y, Zhu L, Zhao Y, Cheng Y, Han L
Science advances 2021 Mar;7(13)

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PRMT5-mediated arginine methylation activates AKT kinase to govern tumorigenesis.

Yin S, Liu L, Brobbey C, Palanisamy V, Ball LE, Olsen SK, Ostrowski MC, Gan W
Nature communications 2021 Jun 8;12(1):3444

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High PRMT5 expression is associated with poor overall survival and tumor progression in bladder cancer.

Tan L, Xiao K, Ye Y, Liang H, Chen M, Luo J, Qin Z
Aging 2020 May 11;12(9):8728-8741

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The LINC01138 drives malignancies via activating arginine methyltransferase 5 in hepatocellular carcinoma.

Li Z, Zhang J, Liu X, Li S, Wang Q, Di Chen, Hu Z, Yu T, Ding J, Li J, Yao M, Fan J, Huang S, Gao Q, Zhao Y, He X
Nature communications 2018 Apr 20;9(1):1572

---

USP9X regulates centrosome duplication and promotes breast carcinogenesis.

Li X, Song N, Liu L, Liu X, Ding X, Song X, Yang S, Shan L, Zhou X, Su D, Wang Y, Zhang Q, Cao C, Ma S, Yu N, Yang F, Wang Y, Yao Z, Shang Y, Shi L
Nature communications 2017 Mar 31;8:14866

Western blot

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

HepG2 cells were subjected to SDS PAGE followed by western blot with 18436-1-AP(PRMT5 antibody) at dilution of 1:800

Sample type

cell line

Immunohistochemistry

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

The PRMT5 antibody from Proteintech is a rabbit polyclonal antibody to a recombinant protein of human PRMT5. This antibody recognizes human,mouse,rat antigen. The PRMT5 antibody has been validated for the following applications: ELISA, WB, IHC analysis.

Appendix

18436-1-AP.pdf

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