NBP2-43678

antibody from Novus Biologicals

Targeting: PRMT5 HRMT1L5, SKB1, SKB1Hs

Western blot (Supportive data in Antibodypedia)

Immunoprecipitation (Supportive data in Antibodypedia)

Antibody Data

Product number

NBP2-43678

Provider

Novus Biologicals

Product name

Mouse Monoclonal PRMT5 Antibody

Antibody type

Monoclonal

Description

Protein G purified.

Reactivity

Human, Rat

Host

Mouse

Isotype

IgG

Vial size

0.1 ml

Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: PRMT5 Antibody (3610) [NBP2-43678] - Analysis of A. 30 ug Jurkat whole cell lysate/extract B. 30 ug Raji whole cell lysate/extract C. 30 ug K562 whole cell lysate/extract D. 30 ug THP-1 whole cell lysate/extract E. 30 ug HL-60 whole cell lysate/extract F. 30 ug NCI-H929 whole cell lysate/extract 7.5 % SDS-PAGE PRMT5 antibody dilution: 1:1000

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: PRMT5 Antibody (3610) [NBP2-43678] - Analysis of A. 50 ug rat kidney lysate/extract 7.5 % SDS-PAGE PRMT5 antibody [3610] dilution: 1:1000.

Immunoprecipitation

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunoprecipitation: PRMT5 Antibody (3610) [NBP2-43678] - Analysis of Raji whole cell extract A. 35 ug Raji whole cell extract B. Control with 4 ug of preimmune Rabbit IgG C. Immunoprecipitation of PRMT5 protein by 4 ug PRMT5 antibody [3610] 7.5 % SDS-PAGE The immunoprecipitated PRMT5 protein was detected by PRMT5 antibody [3610] diluted at 1:1000. [EasyBlot anti-rabbit IgG was used as a secondary reagent]