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antibody from Invitrogen Antibodies
Targeting: PRMT5
HRMT1L5, SKB1, SKB1Hs
Western blot
Immunocytochemistry Immunoprecipitation Immunohistochemistry Chromatin Immunoprecipitation Other assayAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Immunocytochemistry [1]
- Chromatin Immunoprecipitation [2]
- Other assay [1]
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- Product number
- PA5-78323 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- PRMT5 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Positive Control: 293T, A431, Jurkat, Raji Predicted Reactivity: Mouse (98%), Rat (97%), Pig (98%), Rhesus Monkey (93%), Chimpanzee (99%), Bovine (98%) Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.31 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of PRMT5 was performed using HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with PRMT5 Polyclonal Antibody (Product # PA5-78323) at 1:200 dilution in 0.1% BSA and incubated overnight at 4 degree and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, Alexa Fluor® 488 (Product # A27034, 1:2000 dilution) for 45 minutes at room temperature (Panel a: green) in HeLa cells. Nuclei (Panel b: blue) were stained with ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image of HeLa cells showing nucleoplasm and cytosol for PRMT5. Panel e represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Chromatin immunoprecipitation analysis of PRMT5 in K562 chromatin extract using 5 µg of sample. Immunoprecipitation was performed using PRMT5 polyclonal antibody (Product # PA5-78323). Analysis of the precipitated DNA was done by PCR using primer targeting γ-globin promoter.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Cross-linked ChIP was performed with K562 chromatin extract and 5 µg of either control rabbit IgG or PRMT5 Polyclonal Antibody (Product # PA5-78323). The precipitated DNA was detected by PCR with primer set targeting to gamma-globin promoter.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- PRMT5 Polyclonal Antibody immunoprecipitates PRMT5 protein in IP experiments. IP samples: Raji whole cell extract. A. 35 µg Raji whole cell extract. B. Control with 4 µg of preimmune Rabbit IgG. C. Immunoprecipitation of PRMT5 protein by 4 µg PRMT5 Polyclonal Antibody (Product # PA5-78323). 7.5 % SDS-PAGE. The immunoprecipitated PRMT5 protein was detected by PRMT5 Polyclonal Antibody (Product # PA5-78323) diluted at 1:500.
